Comparison of the dimensions of hippocampal CA1 dendritic spines with cerebellar dendritic spines

Range Table - link
Organism Rat Rattus norvegicus
Reference KM Harris and JK Stevens, Dendritic spines of CA 1 pyramidal cells in the rat hippocampus: serial electron microscopy with reference to their biophysical characteristics, The Journal of Neuroscience, 1 August 1989, 9(8): 2982-2997 p.2991 table 4
Primary Source Harris KM, Stevens JK. Dendritic spines of rat cerebellar Purkinje cells: serial electron microscopy with reference to their biophysical characteristics. J Neurosci. 1988 Dec8(12):4455-69.PubMed ID3199186
Method Abstract:"Serial electron microscopy and 3-D reconstructions of dendritic spines from hippocampal area CA 1 dendrites were obtained to evaluate 2 questions about relationships between spine geometry and synaptic efficacy."
Comments p.2989 left column bottom paragraph: "Hippocampal series 17, 18 were from the same animal as cerebellar series 20, and hippocampal series 21, 22, 24 were from the same animal as cerebellar series 25. Therefore, the pH and temperature of the fixative, perfusion times, and processing for electron microscopy were equal. However, fixation quality of the dendrites may not be equivalent in these 2 brain regions because the density of blood vessels in the arachnoid surrounding the Purkinje cell dendrites in the cerebellar folia is much greater than the amount that is found in the hippocampal region. Although membrane structure, microtubule continuity, and mitochondrial integrity were equivalently well preserved in the hippocampus and cerebellum, it is possible that a more rapid perfusion of cerebellum could account for the uniformly continuous SER [Smooth Endoplasmic Reticulum] found in cerebellar dendritic spines. With this caution in mind, a comparison of spine dimensions in the 2 regions is presented in Table 4." See notes beneath table
Entered by Uri M
ID 111664