Identification of total proteins in A. baumannii DU202 cultured in LB medium supplemented with imipenem

Range Table - link
Organism Bacteria Acinetobacter baumannii
Reference Yun SH et al., Quantitative proteomic analysis of cell wall and plasma membrane fractions from multidrug-resistant Acinetobacter baumannii. J Proteome Res. 2011 Feb 4 10(2):459-69. doi: 10.1021/pr101012s. p.462 table 1PubMed ID21053951
Method p.462 left column bottom paragraph:"Total Protein Identification in A. baumannii DU202. Before comparative proteomic analysis was performed under antibiotic stress conditions, [investigators] tried to identify the total proteome of A. baumannii DU202 cultured in LB medium supplemented with imipenem. The soluble protein (cytosol) fraction was prepared by centrifugation after cell disruption, and the membrane-associated (cell wall and membrane) protein fraction was prepared by sodium carbonate precipitation. Proteins from the two fraction types were separated by SDS-PAGE (Figure 1A). The results of the 1-DE-LC/MS-MS [one-dimensional electrophoresis- liquid chromatography-tandem mass spectrometry] analysis are summarized in Table 1."
Comments p.462 left column bottom paragraph:"In total, 597 and 248 proteins were identified from the soluble and membrane-associated fractions, respectively. Of these, 146 proteins were simultaneously identified from both fractions thus, the total number of identified proteins was 699, which represents about 18.4% of the total genome of A. baumannii."
Entered by Uri M
ID 111523