Comparison of metabolic reaction fluxes of amino acids biosynthetic reactions

Range Table - link mmol/gDCW/h
Organism Bacteria Escherichia coli
Reference Selvarasu S. et al., Characterizing Escherichia coli DH5alpha growth and metabolism in a complex medium using genome-scale flux analysis. Biotechnol Bioeng. 2009 Feb 15 102(3):923-34. doi: 10.1002/bit.22119. p.929 table IPubMed ID18853410
Method "Amino acid analysis was done according to the procedure of Cohen and Michaud (1993) using reversed-phase HPLC on a Shimadzu VP-ODS column (4.6 mm250 mm) and fluorescence detection at the respective excitation and emission wavelength of 250 and 395 nm."
Comments "The fermentation culture was mainly explored during three distinct growth phases: an initial exponential growth phase characterized by high growth rate (phase 1, 1–3 h), late exponential growth phase (phase 2, 4–6 h) and acetate consumption phase (early stationary phase: phase 3, 8–10 h) in which acetate was consumed as major carbon source. In silico flux analysis was conducted for all the three phases, among which the metabolic behavior of the first two phases is elaborated in this section (see later sections for phase 3). The specific consumption rates of all measured nutrients during phase 1 and phase 2 were ranked and shown in Figure 2...Obviously, due to the uptake of amino acids from the complex medium, the biosynthetic requirement for protein synthesis could be partially fulfilled, thus resulting in lower fluxes for amino acid biosynthesis relative to the simulated minimal medium condition (Table I)."
Entered by Uri M
ID 111288