| Comments |
"The fermentation culture was mainly explored during
three distinct growth phases: an initial exponential growth
phase characterized by high growth rate (phase 1, 1–3 h),
late exponential growth phase (phase 2, 4–6 h) and acetate
consumption phase (early stationary phase: phase 3, 8–10 h)
in which acetate was consumed as major carbon source. In
silico flux analysis was conducted for all the three phases,
among which the metabolic behavior of the first two phases
is elaborated in this section (see later sections for phase 3).
The specific consumption rates of all measured nutrients
during phase 1 and phase 2 were ranked and shown in
Figure 2...Obviously, due to the uptake of amino
acids from the complex medium, the biosynthetic requirement
for protein synthesis could be partially fulfilled, thus
resulting in lower fluxes for amino acid biosynthesis relative
to the simulated minimal medium condition (Table I)." |