Diffusion coefficient of mRNAs within the nucleus measured by single molecule tracking experiments

Range for a large dystrophin mRNA 0.005: for a small beta-actin mRNA 3.4 μm^2/s
Organism Eukaryotes
Reference Oeffinger M, Zenklusen D. To the pore and through the pore: a story of mRNA export kinetics. Biochim Biophys Acta. 2012 Jun1819(6):494-506. doi: 10.1016/j.bbagrm.2012.02.011. p.497 left column 2nd paragraphPubMed ID22387213
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Method Single molecule tracking experiments
Comments "However, these numbers [obtained by labeling all mRNAs simultaneously, BNID 110665, 110666] were challenged by single molecule tracking [SMT] experiments that allowed following individual mRNAs in the nucleus and, at least in part, suggested a much slower mRNA diffusion rate. Using different labeling approaches (MS2 system, anti-sense oligos and molecular beacons), these SMT experiments estimated diffusion speeds varying from 0.005 µm^2/s for a large dystrophin mRNA to 3.4 µm^2/s for a small beta-actin mRNA [primary sources]."
Entered by Uri M
ID 110667