Average turnover rate of individual stress fibres (actin) as measured by incorporation of fluorescently labelled actin after injection

Range 3.3 to 4.0 min
Organism Mammals
Reference Theriot JA, Mitchison TJ. Actin microfilament dynamics in locomoting cells. Nature. 1991 Jul 11 352(6331):126-31. doi:10.1038/352126a0 p.128 right column top paragraphPubMed ID2067574
Primary Source Amato PA, Taylor DL. Probing the mechanism of incorporation of fluorescently labeled actin into stress fibers.J Cell Biol. 1986 Mar102(3):1074-84.PubMed ID3949874
Method "Incorporation of fluorescently labelled actin after injection"
Comments "The average turnover rate is consistent with the rate in fibroblasts measured by incorporation of fluorescently labelled actin after injection (3.3 to 4.0min, primary source), but is faster than the rate measured by fluorescence recovery after photobleaching about 10 min (ref 20)." Primary source investigated 3T3 (mouse) and WI38 (human) fibroblasts.
Entered by Uri M
ID 109825