Protein copy numbers per HeLa cell

Range Table - link Protein/cell
Organism Human Homo sapiens
Reference Zeiler M, Straube WL, Lundberg E, Uhlen M, Mann M. A Protein Epitope Signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines. Mol Cell Proteomics. 2012 Mar11(3):O111.009613. doi: 10.1074/mcp.O111.009613. p.9 table IIPubMed ID21964433
Method stable isotope labeling of amino acids in cell culture (SILAC)
Comments "Given the known amount of each PrEST (Protein Epitope Signature Tags) and their SILAC ratios with respect to the endogenous proteins we determined the cellular copy numbers of 40 different proteins. Very high accuracy of absolute quantification to within a standard error of 25% was achieved for 35 of 40 proteins (Table II)."
Entered by Uri M
ID 108912