Beam waist of laser beam (420nm) directed at cell nucleus

Value 1.4 µm
Organism Mammalian tissue culture cell
Reference Skovsen E, Snyder JW, Lambert JD, Ogilby PR. Lifetime and diffusion of singlet oxygen in a cell. J Phys Chem B. 2005 May 12 109(18):8570-3. p.8571 left column top paragraphPubMed ID16852012
Method A given cell was irradiated using a femtosecond pulsed laser that was focused into the sample through the microscope objective. Value calculated from the Rayleigh equation using ?=420 nm, diameter ~2×0.61?/NAeff=1.4µm.
Comments Along the axis of propagation, the beam diverges such that at a distance of 10µm from the waist its cross-sectional diameter is ~6µm. Nuclei of neurons from the hippocampus of Wistar rats were irradiated.
Entered by Uri M
ID 105993