| Range |
170-230 Copies/cell
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Jishage M, Ishihama A. Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of sigma 70 and sigma 38. J Bacteriol. 1995 Dec177(23):6832-5.PubMed ID7592475
|
| Method |
For measurement of s subunit levels in cell extracts, a
quantitative Western blot system was developed by using monospecific
polyclonal antibodies against each s subunit. Researchers found that in stationary phase levels of s38 are 30% of s70. Based on s70 levels (500-700 copies/cell, BNID 105160) they arrive at 170-230 copies of s38/cell. |
| Comments |
Maximum levels of s38 were at stationary phase, 30 fmol/µg of total protein |
| Entered by |
Uri M |
| ID |
105161 |