Length of photocycle of proteorhodopsin
| Value | 15 msec |
|---|---|
| Organism | Generic |
| Reference | Béjà O, Aravind L, Koonin EV, Suzuki MT, Hadd A, Nguyen LP, Jovanovich SB, Gates CM, Feldman RA, Spudich JL, Spudich EN, DeLong EF. Bacterial rhodopsin: evidence for a new type of phototrophy in the sea. Science. 2000 Sep 15 289(5486):1902-6.PubMed ID10988064 |
| Method | bacterial rhodopsin was encoded in the genome of an uncultivated gamma-proteobacterium and shared highest amino acid sequence similarity with archaeal rhodopsins. The protein was functionally expressed in Escherichia coli and bound retinal to form an active, light-driven proton pump. The new rhodopsin exhibited a photochemical reaction cycle with intermediates and kinetics characteristic of archaeal proton-pumping rhodopsins. |
| Comments | The decay of proteorhodopsin O is the rate limiting step in the photocycle and is fit well by a single exponential process of 15 msec with an upward baseline shift of 13% of the initial amplitude. A possible explanation is heterogeneity in the proteorhodopsin population, with 87% of the molecules exhibiting a 15 ms photocycle and 13% a slower recovery |
| Entered by | Uri M |
| ID | 103732 |