Fraction of mutations that are lethal

Range ~1 %
Organism Bacteria Escherichia coli
Reference Lydia Robert, Jean Ollion, Jerome Robert, Xiaohu Song, Ivan Matic, and Marina Elez, Mutation dynamics and fitness effects followed in single cells, Science 16 Mar 2018: Vol. 359, Issue 6381, pp. 1283-1286 DOI: 10.1126/science.aan0797 p.1285 left column bottom paragraph
Method P.1285 left column bottom paragraph: "To further disentangle age- and mutation-related mortality, [investigators] performed a µMA [microfluidic mutation accumulation] experiment using the strain pMQ, which has increased proofreading deficiency compared with MF1 (ref 21). pMQ cells died rapidly, and their survival decreased exponentially (Fig.4B, right, and fig. S11)."
Comments P.1285 left column bottom paragraph: "These analyses [see Measurement Method section] demonstrate that lethal mutations occur at a constant rate in µMA, independently of previously acquired mutations, and that ~1% of mutations are lethal."
Entered by Uri M
ID 114862