Average molecular masses of proteins for different organs

Range Figure - link kDa
Organism Mouse Mus musculus
Reference Wiśniewski JR, Hein MY, Cox J, Mann M. A "proteomic ruler" for protein copy number and concentration estimation without spike-in standards. Mol Cell Proteomics. 2014 Dec13(12):3497-506. doi: 10.1074/mcp.M113.037309. p.3504 figure 6BPubMed ID25225357
Method Approximate values extracted visually from figure 6B are (in kDa): Eye 32, Heart 40, Leg 57, Lung 29, Ovary 32, Pituitary 29, prostate 33, thyroid 40.
Comments P.3503 right column bottom paragraph: "The Muscle Proteome Is Quantitatively Dominated by Large, Abundant Proteins: As a practical example of the usefulness of “easy” absolute protein quantification, [investigators] determined cell sizes and cellular copy numbers of proteins in a panel of other mouse organs (Fig. 6A BNID 113240). Ovaries consist predominantly of small follicular cells and showed the least protein per cell (42 pg). Leg muscle cells, in contrast, had around 675 pg of protein per nucleus. Considering that muscle fibers are syncytial, multi-nucleated cells, the histone proteomic ruler delivered protein amounts per nucleus and not per cell in this particular case. Despite the huge differences in cellular protein amounts, [they] observed much less variation in the dependence of the abundance of a protein and its molecular mass, irrespective of the tissue of origin. This is reflected in the average molecular mass of a protein, which is calculated as the ratio of the total protein mass per cell to the total number of protein molecules (Fig. 6B). This number is rather similar across tissues, with the notable exception of muscle tissues. The reason for this becomes apparent when [they] look at the distribution of protein sizes across the dynamic range of the individual proteins (Figs. 6C and 6D)."
Entered by Uri M
ID 113241