| Range |
Table - link protein copies/cell
|
| Organism |
Human Homo sapiens |
| Reference |
Wisniewski JR, Ostasiewicz P, Dus K, Zielinska DF, Gnad F, Mann M. Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma. Mol Syst Biol. 2012 8: 611. doi: 10.1038/msb.2012.44. p.9 table IVPubMed ID22968445
|
| Primary Source |
See pointers to refs beneath table |
| Method |
"To survey and compare the proteomes of colonic mucosa
(normal or N) and colon cancer (cancer or C), [researchers] analyzed
archival FFPE [formalin-fixed and paraffin-embedded] clinical samples originating from eight patients
(Supplementary Table 1 Materials and methods). In addition,
for seven of the patients [researchers] analyzed the proteomes of nodal
metastases (M). Laser capture microdissection was used to
obtain enriched populations of enterocytes, primary cancer,
and metastasizing cells (Supplementary Figure 1). [They] made an
effort to reduce any contamination by stroma and therefore did
not emphasize identification of secreted proteins. From each
sample, a volume of about 175 nl of cells were collected and
processed using the FFPE-FASP (filter aided sample preparation)
procedure (Wisniewski et al, 2011a)." |
| Comments |
"Core histones are among the most abundant proteins in the
eukaryotic cells. [Researchers'] copy numbers for these proteins were 1.1×10^8 in normal mucosa to 4.4×10^8 in undifferentiated
CaCo-2 cells (Table IV)...Western blot-based quantitative characterization of RNA
polymerase II complex in HeLa cells by Kimura et al (1999)
revealed that its subunits are present at 1.5–3.0×10^5 copies
per cell (Table IV)." See notes beneath table |
| Entered by |
Uri M |
| ID |
111218 |