H+/P (H+/ATP) ratio during hydrolysis of added ATP a in both mitochondria and submitochondrial particles

Value 2 unitless
Organism Eukaryotes
Reference Brand MD, Lehninger AL. H+/ATP ratio during ATP hydrolysis by mitochondria: modification of the chemiosmotic theory. Proc Natl Acad Sci U S A. 1977 May74(5):1955-9. p.1955 left column bottom paragraphPubMed ID17116
Primary Source 7) Mitchell, P. & Moyle, J. (1965) Stoichiometry of proton translocation through the respiratory chain and adenosine triphosphatase systems of rat liver mitochondria. Nature 208, 147-151. (10) Bielawski, J. & Lehninger, A. L. (1966) Stoichiometric relationships in mitochondrial accumulation of calcium and phosphate supported by hydrolysis of adenosine triphosphate. J. Biol. Chem. 241, 4316-4322. (11) Rossi, C. S., Siliprandi, N., Carafoli, E., Bielawski, J. & Lehninger, A. L. (1967) Proton movements across the mitochondrial membrane supported by hydrolysis of adenosine triphosphate. Eur. J. Biochem. 2, 332-340. (12) Mitchell, P. & Moyle, J. (1968) Proton translocation coupled to ATP hydrolysis in rat liver mitochondria. Eur. J. Biochem. 4,530-539. (13) Mitchell, P. & Moyle, J. (1970) Influence of aurovertin on affinity of mitochondrial adenosine triphosphatase for ATP and ADP. FEBS Lett. 6, 309-311. (14) Moyle, J. & Mitchell, P. (1973) Proton translocation quotient for the adenosine triphosphatase of rat liver mitochondria. FEBS Lett. 30, 317-320. (15) Thayer, W. S. & Hinkle, P. C. (1973) Stoichiometry of adenosine triphosphate-driven proton translocation in bovine heart submitochondrial particles. J. Biol. Chem. 248, 5395-5402.PubMed ID4222981, 4162498, 4965707, 4232392, 11947402, 4267054, 4358615
Comments "The original measurements of the H+/site ratio by Mitchell and Moyle (7, 8) yielded the value 2.0 (see also ref. 9). Since measurements of the H+/ATP ratio during hydrolysis of added ATP also gave the value 2.0 in both mitochondria (primary sources 7, 10-14) and submitochondrial particles (primary sources 14, 15), the postulated condition of equality (H+/site = H+/ATP) was apparently fulfilled."
Entered by Uri M
ID 110657