| Value |
50
µM
Range: ±10 µM
|
| Organism |
Unspecified |
| Reference |
Bernstein BW, Bamburg JR. Actin-ATP hydrolysis is a major energy drain for neurons. J Neurosci. 2003 Jan 1 23(1):1-6. p.2 right column 2nd paragraphPubMed ID12514193
|
| Primary Source |
Gupta RK, Gupta P, Yushok WD, Rose ZB (1983) Measurement of the dissociation constant of MgATP at physiological nucleotide levels by a combination of 31PNMRand optical absorbance spectroscopy. Biochem Biophys Res Commun 117: 210–216.PubMed ID6607050
|
| Method |
(primary source abstract:)"The new combination method utilizes 31P NMR chemical shifts to determine the degree of Mg++ chelation of ATP in a solution containing free ATP and MgATP, and uses a properly calibrated indicator dye, antipyrylazo III, for optical measurement of free Mg++ in the same solution." |
| Comments |
"To monitor the loss of ATP in live cells, [researchers] took advantage of
the fact that the affinity of Mg2+ for ATP (Kd=50±10µM) (primary source) is ~10-fold higher than for ADP or AMP
(Leyssens et al., 1996). As ATP is hydrolyzed to ADP and AMP,
[Mg2+]i rises (Budinger et al., 1998)." |
| Entered by |
Uri M |
| ID |
110639 |