Fraction of RNAP found engaged in active transcription

Range ~50 %
Organism Bacteria Escherichia coli
Reference Klumpp S. A superresolution census of RNA polymerase. Biophys J. 2013 Dec 17 105(12):2613-4. doi: 10.1016/j.bpj.2013.11.018. p.2613 middle column bottom paragraphPubMed ID24359730
Primary Source Bakshi, S. et al., 2013. Partitioning of RNA polymerase activity in live E. coli from analysis of single-molecule diffusive trajectories. Biophys. J. 105: 2676–2686. doi: 10.1016/j.bpj.2013.10.024.PubMed ID24359739
Comments "By quantifying these fractions, Bakshi et al. (primary source) obtain a global picture of the activities of RNAPs in the cell. Only approximately one-half of the RNAPs are found engaged in active transcription, but a large fraction is bound to DNA, either specifically or nonspecifically (82%)."
Entered by Uri M
ID 110044