| Range |
100 - 160 Table - link ATP/molecule
|
| Organism |
Mammals |
| Reference |
Peth A, Nathan JA, Goldberg AL. The ATP costs and time required to degrade ubiquitinated proteins by the 26 S proteasome. J Biol Chem. 2013 Oct 4 288(40):29215-22. doi: 10.1074/jbc.M113.482570 p.10 Table 1PubMed ID23965995
|
| Method |
"Initial experiments used 26S proteasomes gently
isolated from yeast (16) to determine whether they
hydrolyze ATP by a stochastic or a highly
cooperative manner, such as the cyclical, ordered
mechanism proposed recently in which nucleotides
bind and exchange in pairs (13)." |
| Comments |
"at
Vmax, the cost of ATP per molecule of Ub5-DHFR
degraded appears to be approximately 50–80 ATP,
while breakdown of Ubn-Sic1 requires ~100–160
ATP (see Table 1)." See note beneath table. |
| Entered by |
Uri M |
| ID |
109852 |