Contribution of SPs (sphingolipids) to the AP (apical membrane preparation) in MDCK cells

Value 13.7 mol% Range: ±0.8 Figure link - link mol%
Organism Mammalian tissue culture cell
Reference Gerl et al., Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane. J Cell Biol. 2012 Jan 23 196(2):213-21 p.215 left column 2nd paragraph and fig2BPubMed ID22249292
Method "[Researchers] used a shotgun lipidomics approach that combines high mass accuracy and resolution mass spectrometry (MS) and a two-step extraction procedure for optimal recovery of polar lipids with the use of internal standard mixtures for absolute quantification of 18 lipid classes and 419 lipid species..."
Comments "Although GPLs [Glycerophospholipids and the glycerolipid DAG] were in general reduced, SPs were enriched in the AP, contributing 13.7 ± 0.8 mol% to its composition."
Entered by Uri M
ID 108433