Volume of bound water in medium of low (0.10 OSM) and moderate (0.28 OSM) osmolarity

Value 0.4 μl/mg dry wt Range: ±0.04 μl/mg dry wt
Organism Bacteria Escherichia coli
Reference Cayley S, Lewis BA, Guttman HJ, Record MT Jr. Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. Implications for protein-DNA interactions in vivo. J Mol Biol. 1991 Nov 20 222(2):281-300 p.281 top paragraphPubMed ID1960728
Comments "Measurements of volume of cytoplasmic water in titrations of non-growing cells with the plasmolyzing agent NaCl were used to obtain volumes of “bound” water (presumably water of macromolecular hydration) and cytoplasmic osmotic coefficients for cells grown in medium of low (0.10 OSM) and moderate (0.28 OSM) osmolarity. The volume of bound water is similar in the two osmotic conditions (volume of bound water=0.40(±O.04)µl/mg dry wt), and corresponds to approximately 0.5g H2O/g cytoplasmic macromolecule."
Entered by Uri M
ID 108416