Number of copies of Ef-Tu during exponential growth

Range ~100,000 molecules per cell
Organism Bacteria Bacillus subtilis
Reference Maass S et al., Efficient, global-scale quantification of absolute protein amounts by integration of targeted mass spectrometry and two-dimensional gel-based proteomics. Anal Chem. 2011 Apr 1 83(7):2677-84. p.2681 left column top paragraphPubMed ID21395229
Method "[Researchers] combined targeted mass spectrometry for the accurate determination of absolute protein abundance of specific proteins with the resolving power of 2-D PAGE to obtain a global view of a large number of protein concentrations."
Comments "During exponential growth the elongation factor Ef-Tu (gene product of tufA) represents the most abundant protein in B. subtilis with about 100,000 molecules per cell."
Entered by Uri M
ID 108409