Myosin I motor speed in vitro

Value 200 nm/sec
Organism Acanthamoeba castellanii
Reference Zot HG, Doberstein SK, Pollard TD. Myosin-I moves actin filaments on a phospholipid substrate: implications for membrane targeting. J Cell Biol. 1992 Jan116(2):367-76. p.369 right column 3rd paragraphPubMed ID1530945
Method The fabrication of a "Kron-style" flow cell, the formation of a film of nitrocellulose on cover slips, and the preparation of basic solutions were as described (Kron et al ., 1991) .
Comments The gliding velocity of actin over myosin-I-coated nitrocellulose varied about a mean of 0.2 µm/s (Fig. 3) reminiscent of muscle myosin, which has a mean velocity of 3-4µm/s (Kron and Spudich, 1986) .
Entered by Uri M
ID 107885