Virion production rate in Monocyte Derived Macrophages and Peripheral Blood Mononuclear Cells

Range 920 in MDM: 5,310 in PBMC virions/cell/day
Organism HIV
Reference Tsai WP, Conley SR, Kung HF, Garrity RR, Nara PL. Preliminary in vitro growth cycle and transmission studies of HIV-1 in an autologous primary cell assay of blood-derived macrophages and peripheral blood mononuclear cells. Virology. 1996 Dec 15 226(2):205-16. p.210 right column and p.211 left columnPubMed ID8955040
Method Cell-free virus production during the stationary period was determined on a per cell basis for both PBMCs and MDMs from the relationship between the cell concentration per T25 flask (15×10^6 total macrophages in 11 ml of medium) and the amount of virus produced per unit time following a complete wash-out of cell-free virus.
Entered by Uri M
ID 105875