Concentration of Rubisco active sites in a crystal

Value 10.7 mM
Organism Spinach Spinacia oleracea
Reference Knight S, Andersson I & Branden CI Crystallographic analysis of ribulose 1,5-bisphosphate carboxylase from spinach at 2.4 A resolution. Subunit interactions and active site. J Mol Biol. 1990 215:113-60PubMed ID2118958
Method X ray crystallography. The X-ray structure of the quaternary complex of ribulose 1,5-bisphosphate carboxylase/oxygenase from spinach with CO2, Mg2+ and a reaction-intermediate analogue (CABP) has been determined and refined at 2.4 A resolution. Cyclic non-crystallographic symmetry averaging around the molecular 4-fold axis and phase combination were used to improve the initial multiple isomorphous replacement phases.
Entered by george
ID 105121