Rate difference for restriction enzyme EcoRI endonuclease activity for cognate and next best site (ideal conditions)

Value 100000 unitless
Organism Generic
Reference link
Primary Source Lesser, D.R., Kurpiewski, M.R. and Jen-Jacobson, L. (1990) The energetic basis of specificity in the EcoR I endonucleaseDNA interaction. Science 250, 776–86.PubMed ID2237428
Comments under ideal conditions. However, under nonoptimal conditions, the differences in cleavage rates between cognate and next-best sites change dramatically for many enzymes. This loss of fidelity or increase in cleavage at sites similar to the cognate site is commonly referred to as star activity.
Entered by Ron Milo - Admin
ID 101485