Number of proteins in cell

Range 3,000,000-4,000,000 proteins/cell
Organism Bacteria Escherichia coli
Reference Milo R. What is the total number of protein molecules per cell volume? A call to rethink some published values. Bioessays. 2013 Dec35(12):1050-5. doi: 10.1002/bies.201300066 p.1053 middle column bottom paragraphPubMed ID24114984
Method Abstract: "Here, [investigators] estimate the expected total number of proteins per unit of cell volume using known parameters related to the composition of cells such as the fraction of cell mass that is protein, and the average protein length. Using simple arguments, [they] estimate a range of 2-4 million proteins per cubic micron (i.e. 1 fL) in bacteria, yeast, and mammalian cells."
Comments P.1053 middle column bottom paragraph: "[Investigators] can now use characteristic volumes to reach the number of proteins per cell. For an E. coli cell of 1 μm^3 volume (average values often vary between 0.5 and 2 μm^3 depending on growth rate and conditions), the estimates give a range of 3–4 million proteins per cell."
Entered by Uri M
ID 115702