||Bacteria Escherichia coli
||Sørensen MA, Fehler AO & Svenningsen SL. Transfer RNA instability as a stress response in Escherichia coli: Rapid dynamics of the tRNA pool as a function of demand. RNA Biol. 2017 Oct 12: 0. doi: 10.1080/15476286.2017.1391440 p.5 2nd paragraphPubMed ID29023189
|| Svenningsen SL et al., Transfer RNA is highly unstable during early amino acid starvation in Escherichia coli. Nucleic Acids Res. 2017 Jan 25 45(2):793-804. doi: 10.1093/nar/gkw1169PubMed ID27903898
||P.5 2nd paragraph: "The spike-in-cell approach has the clear advantage that no assumptions about constancy of the levels of the reference RNA across the tested conditions are necessary, because the spike-in cells are added after harvest of the experimental samples. Addition of the spike-in cells prior to RNA extraction (as opposed to the addition of purified spike-in transcripts after RNA purification) ensures that the reference RNA also reflects any sample-to-sample variation in RNA recovery.
Using the spike-in-cell approach, [investigators] showed that the majority of cellular tRNA is degraded within twenty minutes of the onset of a sudden amino acid starvation (primary source)."