Size of inclusion body and fraction of inclusion body that is heterologously expressed protein

Range size 0.2-1.2µm: fraction that is heterologously expressed protein 80-95%
Organism bacteria
Reference Uversky VN. Intrinsically disordered proteins in overcrowded milieu: Membrane-less organelles, phase separation, and intrinsic disorder. Curr Opin Struct Biol. 2017 Jun44: 18-30. doi: 10.1016/ p.27 right column top paragraphPubMed ID27838525
Primary Source [54] Ramón A et al., Inclusion bodies: not that bad… Front Microbiol. 2014 Feb 14 5: 56. doi: 10.3389/fmicb.2014.00056 p.2 left column bottom paragraphPubMed ID24592259
Comments P.27 right column top paragraph: "In addition to the eukaryotic PMLOs [proteinaceous membrane-less organelles] described in this article, membrane-less organelles known as inclusion bodies (IBs) can be found in some prokaryotic cells, especially in bacteria producing heterologous proteins [primary source]. These bacterial IBs contain aggregated proteins and, therefore, are similar to the eukaryotic aggresomes. In fact, both IBs and aggresomes represent a result of the regulated aggregation that originates from the altered proteostasis caused by the distortion in the cellular protein quality control system that includes chaperones and proteases [primary source]. In bacteria, IBs are found in the cytoplasm as dense spherical or cylindrical particles whose size is ranging from 0.2 to 1.2 μm and whose major constituent (80–95%) is the heterologously expressed protein. However, similar to other PMLOs, IBs may also contain other proteins and nucleic acids [primary source]." Primary source abstract: "The formation of inclusion bodies (IBs) constitute a frequent event during the production of heterologous proteins in bacterial hosts."
Entered by Uri M
ID 114204