Mobility analysis of single BR2 [Balbiani Ring 2] mRNPs and reference particles

Range Table - link
Organism Midge Chironomus tentans
Reference Siebrasse JP et al., Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin. Proc Natl Acad Sci U S A. 2008 Dec 23 105(51):20291-6. doi: 10.1073/pnas.0810692105. p.20293 table 1PubMed ID19074261
Method p.20291 right column 3rd paragraph:"In the present study, [investigators] have analyzed the intranuclear mobility of a single native mRNP species, the BR2 [Balbiani Ring 2] mRNP. Fluorescence labeling was achieved in vivo by microinjection of fluorescent oligonucleotides (DNA or RNA), which were complementary to a highly repetitive coding sequence in BR2 mRNA."
Comments p.20293 left column 3rd paragraph:"[Investigators’] jump-distance analysis was based on the trajectories of 4 independent experiments (11,256 trajectories) comprising almost 50,000 single BR2 mRNP jumps between successive frames. The analysis of these data revealed that there are at least 3 mobility fractions present (I–III) (Fig. 2D and Table 1). The fastest particles exhibited DI = 4.0 ± 1.3 µm^2/s. Two-thirds of the jumps showed DII = 0.7 ± 0.1 µm^2/s (fraction II), and the slowest fraction (III) yielded DIII = 0.24 ± 0.05 µm^2/s. In addition to the jump-distance analysis, [they] performed an MSD [mean-square displacement] analysis that yielded an average diffusion coefficient DMSD = 0.76 ± 0.03 µm^2/s (Fig. 2E)."
Entered by Uri M
ID 111858