| Range |
Table - link mmol/gDCW/h
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Selvarasu S. et al., Characterizing Escherichia coli DH5alpha growth and metabolism in a complex medium using genome-scale flux analysis. Biotechnol Bioeng. 2009 Feb 15 102(3):923-34. doi: 10.1002/bit.22119. p.931 tables IV & VPubMed ID18853410
|
| Method |
"Amino acid analysis was done according to the procedure of
Cohen and Michaud (1993) using reversed-phase HPLC on
a Shimadzu VP-ODS column (4.6 mm250 mm) and
fluorescence detection at the respective excitation and
emission wavelength of 250 and 395 nm." |
| Comments |
"The fermentation culture was mainly explored during
three distinct growth phases: an initial exponential growth
phase characterized by high growth rate (phase 1, 1–3 h),
late exponential growth phase (phase 2, 4–6 h) and acetate
consumption phase (early stationary phase: phase 3, 8–10 h)
in which acetate was consumed as major carbon source. In
silico flux analysis was conducted for all the three phases,
among which the metabolic behavior of the first two phases
is elaborated in this section (see later sections for phase 3).
The specific consumption rates of all measured nutrients
during phase 1 and phase 2 were ranked and shown in
Figure 2." See notes beneath tables |
| Entered by |
Uri M |
| ID |
111290 |