||Lin JC, Thirumalai D. Gene regulation by riboswitches with and without negative feedback loop. Biophys J. 2012 Dec 5 103(11):2320-30. doi: 10.1016/j.bpj.2012.10.026. p.2322 right column top paragraphPubMed ID23283231
||"[Researchers] introduce a kinetic network model that describes the functions of riboswitches at the systems level. Using experimental data for flavin mononucleotide riboswitch as a guide, [they] show that efficient function, implying a large dynamic range without compromising the requirement to suppress transcription, is determined by a balance between the transcription speed, the folding and unfolding rates of the aptamer, and the binding rates of the metabolite."
||"Large excess of metabolite, exceeding the equilibrium KD (~10 nM) for FMN binding to B*2, over RNA transcript is needed for the
metabolites to bind the riboswitches with sufficient speed to regulate transcription under the conditions explored in experiments (ref 9)."