Cut in enzyme dihydrofolate reductase (DHFR) abundance which can be sustained with little effect on fitness

Value 80 %
Organism Bacteria Escherichia coli
Reference Firnberg E, Labonte JW, Gray JJ, Ostermeier M: A Comprehensive, High-Resolution Map of a Gene's Fitness Landscape. Mol Biol Evol 2014. p.7 left columnPubMed ID24567513
Primary Source Bershtein S, Mu W, Serohijos AW, Zhou J, Shakhnovich EI. 2013. Protein quality control acts on folding intermediates to shape the effects of mutations on organismal fitness. Mol Cell. 49:133–144.PubMed ID23219534
Method (primary source abstract:) "[Researchers] mutate the E. coli's gene encoding dihydrofolate reductase (DHFR) and replace it with bacterial orthologous genes to determine how components of PQC [protein quality control] modulate fitness effects of these genetic changes."
Comments "A ... study estimated that DHFR could sustain an 80% cut in protein abundance with little effect on organismal fitness and that the dependence of fitness on protein abundance exhibited Michaelis–Menten-like behavior (primary source)."
Entered by Uri M
ID 110452