Vmax for the degradation of ubiquitinated Ubn-Sic1 by mammalian 26S proteasomes

Value 2.3 molecules/min/26S Range: Table - link molecules/min/26S
Organism Mammals
Reference Peth A, Nathan JA, Goldberg AL. The ATP costs and time required to degrade ubiquitinated proteins by the 26 S proteasome. J Biol Chem. 2013 Oct 4 288(40):29215-22. doi: 10.1074/jbc.M113.482570 p.10 Table 1PubMed ID23965995
Method "Initial experiments used 26S proteasomes gently isolated from yeast (16) to determine whether they hydrolyze ATP by a stochastic or a highly cooperative manner, such as the cyclical, ordered mechanism proposed recently in which nucleotides bind and exchange in pairs (13)."
Comments See note beneath table.
Entered by Uri M
ID 109855