Ratio of free/bound cytoplasmic water

Range ~4.5 in E. coli grown at 0.1 Osm: ~1.5 at 1 Osm unitless
Organism Bacteria Escherichia coli
Reference Cayley S, Record MT Jr. Large changes in cytoplasmic biopolymer concentration with osmolality indicate that macromolecular crowding may regulate protein-DNA interactions and growth rate in osmotically stressed Escherichia coli K-12. J Mol Recognit. 2004 Sep-Oct17(5):488-96. p.490 right column top paragraphPubMed ID15362109
Primary Source Cayley S, Record MT Jr. Roles of cytoplasmic osmolytes, water, and crowding in the response of Escherichia coli to osmotic stress: biophysical basis of osmoprotection by glycine betaine. Biochemistry. 2003 Nov 4 42(43):12596-609.PubMed ID14580206
Comments "Interpreted according to the two-domain model of water, researchers' measurements show that the ratio of free/bound cytoplasmic water decreases from ~4.5 in E. coli grown at 0.1 Osm to ~1.5 at 1 Osm, and indicate that the amount of free water approaches zero at the osmolality where growth rate approaches zero (primary source)."
Entered by Uri M
ID 108396