Frequencies of transfer of marker genes to both cultured and natural communities

Range Table - link cfu^-1
Organism Bacteria Roseovarius nubinhibens
Reference McDaniel LD, Young E, Delaney J, Ruhnau F, Ritchie KB, Paul JH. High frequency of horizontal gene transfer in the oceans. Science. 2010 Oct 1330(6000):50. table 1PubMed ID20929803
Method To document gene transfer frequencies, researchers subjected wild-type strains or natural communities from a range of environments to treatment with donor strain GTAs (Gene Transfer Agent) and documented the rates of GTA-mediated gene transfer of kanamycin resistance. The gene transfer frequencies were calculated by dividing the number of antibiotic resistant cells by the total number of recipient cells in the culture (S6), both measured as colony-forming units (cfu)/ml.
Entered by Uri M
ID 105849