Turnover rate of GAP (Ras GTPase activating protein) in PC12 cells

Value 720 min^-1 Range: Table - link min^-1
Organism Rat Rattus norvegicus
Reference Brightman FA and Fell DA, FEBS Lett. Differential feedback regulation of the MAPK cascade underlies the quantitative differences in EGF and NGF signalling in PC12 cells. 2000 Oct 6 482(3):169-74 p. 171 table 1PubMed ID11024454
Primary Source Gideon P, John J, Frech M, Lautwein A, Clark R, Scheffler JE, Wittinghofer A. Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: the C-terminal domain of GAP is not sufficient for full activity. Mol Cell Biol. 1992 May12(5):2050-6. AND Ahmadian MR, Hoffmann U, Goody RS, Wittinghofer A. Individual rate constants for the interaction of Ras proteins with GTPase-activating proteins determined by fluorescence spectroscopy. Biochemistry. 1997 Apr 15 36(15):4535-41PubMed ID1569940, 9109662
Method (primary source 2) stopped flow experiments using fluorescent derivatives of guanine nucleotides
Entered by Uri M
ID 105647