|Budding yeast Saccharomyces cerevisiae
|Zadrag-Tecza R, Kwolek-Mirek M, Bartosz G, Bilinski T. Cell volume as a factor limiting the replicative lifespan of the yeast Saccharomyces cerevisiae. Biogerontology. 2008 Nov 5. Table 2PubMed ID18985429
|Cell volume was estimated by analysis of microscopic images recorded every each five cell divisions during a routine procedure of determination of replicative lifespan. Images were captured with a Nikon Eclipse E200 microscope with 20× objective, equipped with a Sony digital camera. Diameter of the cell was measured using a MicroImage 3.0 software. Cell diameter (d) was measured in four times in various planes for each cell and the mean value was used for calculations. It was assumed that each cell has a regular shape similar to a sphere and the cell volume was calculated as 4/3p(d/2)3.
|In a population, cells cease to bud after various number of cell cycles. The ratio of volumes of the bud and the mother cell in the wild-type strains studied was 0.50–0.70 in the first budding decreasing to about 0.28 in the last buddings.