Conductance rate of Glycerol conducting channel

Value 5e+8 sec^-1
Organism Bacteria Escherichia coli
Reference Stroud RM, Miercke LJ, O'Connell J, Khademi S, Lee JK, Remis J, Harries W, Robles Y, Akhavan D. Glycerol facilitator GlpF and the associated aquaporin family of channels. Curr Opin Struct Biol. 2003 Aug13(4):424-31.PubMed ID12948772
Primary Source M.J. Borgnia, D. Kozono, G. Calamita, P.C. Maloney and P. Agre, Functional reconstitution and characterization of AqpZ, the E. coli water channel protein. J Mol Biol 291 (1999), pp. 1169–1179.PubMed ID10518952
Method The permeability of the liposomes to small solutes was performed by rapidly mixing a volume of suspension vesicles at a given concentration of permeant solute with a volume of solution with the osmolarity equilibrated with a non-permeant solute. The effective concentration of the solute in the outer medium is then reduced to half, generating an outward gradient. The outward movement of permeant solute generates an outwardly oriented osmotic gradient, which drives water movement.
Entered by Uri M
ID 103683