Maximum turn over rate of Amplex Red by Horseradish peroxidase

Value 710 sec^-1
Organism Generic
Reference Gorris HH, Walt DR. Mechanistic aspects of horseradish peroxidase elucidated through single-molecule studies. J Am Chem Soc. 2009 May 6131(17):6277-82PubMed ID19338338
Method Enclosing a population of hundreds of single enzyme molecules in a large array of femtoliter reaction chambers and observing the individual molecules by fluorescence microscopy
Comments HRP first oxidizes fluorogenic substrate molecules like Amplex Red to radical intermediates. Two radical molecules subsequently undergo an enzyme-independent dismutation reaction
Entered by Uri M
ID 104239