Glutamine levels in E. coli grown in chemostat

Range 0.1-10 mM
Organism Bacteria Escherichia coli
Reference Senior PJ. Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with the continuous-culture technique. J Bacteriol. 1975 Aug123(2):407-18.PubMed ID238954
Method A mechanically coupled NBS Microferm fermentor was used in conjunction with a 5-liter (2-liter working volume) culture vessel adapted for continuous-culture operation by the incorporation of a wier into the harvest line such that effluent gas and culture were driven out of the vessel down a common line.
Entered by Uri M
ID 103886