Length of cycle in which expression of ASCL1 oscillates in undifferentiated progenitors in the neural tube

Range 2-3 hours
Organism Mouse Mus musculus
Reference Huang C, Chan JA, Schuurmans C. Proneural bHLH genes in development and disease. Curr Top Dev Biol. 2014 110: 75-127. doi: 10.1016/B978-0-12-405943-6.00002-6 p.97 bottom paragraphPubMed ID25248474
Primary Source Castro DS et al., Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif. Dev Cell. 2006 Dec11(6):831-44 DOI: 10.1016/j.devcel.2006.10.006 AND Castro DS et al., A novel function of the proneural factor Ascl1 in progenitor proliferation identified by genome-wide characterization of its targets. Genes Dev. 2011 May 1 25(9):930-45. doi: 10.1101/gad.627811 AND Imayoshi I et al., Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. Science. 2013 Dec 6 342(6163):1203-8. doi: 10.1126/science.1242366 AND Shimojo H, Ohtsuka T, Kageyama R. Oscillations in notch signaling regulate maintenance of neural progenitors. Neuron. 2008 Apr 10 58(1):52-64. doi: 10.1016/j.neuron.2008.02.014PubMed ID17141158, 21536733, 24179156, 18400163
Method Primary source Castro et al. 2006 Abstract: "[Investigators] identify by in silico screening a number of additional candidate target genes…" Primary source Castro et al. 2011 Abstract: "[Investigators] performed a genome-wide characterization of the transcriptional targets of Ascl1 in the embryonic brain and in neural stem cell cultures by location analysis and expression profiling of embryos overexpressing or mutant for Ascl1." Primary source Imayoshi et al. abstract: "Here, [investigators] found by time-lapse imaging that these factors [the basic helix-loop-helix transcription factors Ascl1/Mash1, Hes1, and Olig2] are expressed in an oscillatory manner by mouse neural progenitor cells… [They] used optogenetics to control expression of Ascl1 and found that, although sustained Ascl1 expression promotes neuronal fate determination, oscillatory Ascl1 expression maintains proliferating neural progenitor cells." Primary source Shimojo et al. abstract: "Here, by using a real-time imaging method, [investigators] found that Hes1 expression dynamically oscillates in neural progenitors."
Comments P.97 bottom paragraph: "Ascl1 specifies distinct neuronal phenotypes in different regions of the neural tube. Similar to Neurog2, Ascl1 expression also oscillates in 2–3 h cycles in undifferentiated progenitors due to its ability to transactivate the Notch ligand Dll1 and initiate Notch-mediated lateral inhibition (primary sources)."
Entered by Uri M
ID 117245