Age (of cell) in which newly generated granule cells begin to make functional afferent and efferent connections (after having migrated into the granule cell layer of the DG (dentate gyrus, in hippocampus)) in adult animals

Range ~2 weeks of age of granule cell
Organism Mouse Mus musculus
Reference Josselyn SA, Frankland PW. Infantile amnesia: a neurogenic hypothesis. Learn Mem. 2012 Aug 16 19(9):423-33. doi: 10.1101/lm.021311.110 p.430 left column bottom paragraphPubMed ID22904373
Primary Source Zhao C et al., Distinct morphological stages of dentate granule neuron maturation in the adult mouse hippocampus. J Neurosci. 2006 Jan 4 26(1):3-11 DOI: 10.1523/JNEUROSCI.3648-05.2006 AND Toni N et al., Synapse formation on neurons born in the adult hippocampus. Nat Neurosci. 2007 Jun10(6):727-34 DOI: 10.1038/nn1908 AND Toni N et al., Neurons born in the adult dentate gyrus form functional synapses with target cells. Nat Neurosci. 2008 Aug11(8):901-7. doi: 10.1038/nn.2156PubMed ID16399667, 17486101, 18622400
Method Primary source Zhao et al. abstract: "By using retrovirus-mediated gene transduction, [investigators] followed the dendritic and axonal growth of adult-born neurons in the mouse dentate gyrus and identified distinct morphological stages that may indicate different levels of connectivity." Primary source Toni et al. 2007 abstract: "Here [investigators] explored the mechanisms of synaptogenesis on neurons born in the adult mouse hippocampus using confocal microscopy, electron microscopy and live imaging." Primary source Toni et al. 2008 abstract: "By combining retroviral expression of green fluorescent protein in adult-born neurons of the mouse dentate gyrus with immuno-electron microscopy, [investigators] found output synapses that were formed by labeled terminals on appropriate target cells in the CA3 [Cornu Ammonis region 3 (in hippocampus)] area and the hilus. Furthermore, retroviral expression of channelrhodopsin-2 allowed [them] to light-stimulate newborn granule cells and identify postsynaptic target neurons by whole-cell recordings in acute slices."
Comments P.430 left column bottom paragraph: "The two phases of neurogenesis depend on the age of the adult-generated neurons. Shortly after they are born, newly generated granule cells migrate into the granule cell layer of the DG, and by ∼2 wk they begin to make functional afferent and efferent connections (primary sources)."
Entered by Uri M
ID 117233