Fraction of neurons in the striatum that are cholinergic interneurons

Range ~2 % of the neurons
Organism Mammals
Reference Goldberg JA, Reynolds JN. Spontaneous firing and evoked pauses in the tonically active cholinergic interneurons of the striatum. Neuroscience. 2011 Dec 15 198: 27-43. doi: 10.1016/j.neuroscience.2011.08.067 p.29 left column 3rd paragraphPubMed ID21925242
Primary Source Kemp JM, Powell TP. The synaptic organization of the caudate nucleus. Philos Trans R Soc Lond B Biol Sci. 1971 Sep 30 262(845):403-12 DOI: 10.1098/rstb.1971.0103 AND Bolam JP, Wainer BH, Smith AD. Characterization of cholinergic neurons in the rat neostriatum. A combination of choline acetyltransferase immunocytochemistry, Golgi-impregnation and electron microscopy. Neuroscience. 1984 Jul12(3):711-8. DOI: 10.1016/0306-4522(84)90165-9 AND Phelps PE, Houser CR, Vaughn JE. Immunocytochemical localization of choline acetyltransferase within the rat neostriatum: a correlated light and electron microscopic study of cholinergic neurons and synapses. J Comp Neurol. 1985 Aug 15 238(3):286-307 DOI: 10.1002/cne.902380305PubMed ID4399121, 6382048, 4044917
Method Primary source Bolam et al., abstract: "Immunocytochemistry with a monoclonal antibody against choline acetyltransferase has been used to characterise cholinergic neurons in the rat neostriatum. The light microscopic morphology, ultrastructure and synaptic input of these neurons was compared to that of the three types of large neuron found in Golgi preparations of the striatum… Examination in the electron microscope revealed that the synaptic input to perikarya and proximal dendrites is sparse…" Primary source Phelps et al., abstract: "Monoclonal antibodies to choline acetyltransferase (ChAT) were used in an immunocytochemical study to characterize putative cholinergic neurons and synaptic junctions in rat caudate-putamen. Light microscopy (LM) revealed that ChAT-positive neurons are distributed throughout the striatum. These cells have large oval or multipolar somata, and exhibit three to four primary dendrites that branch and extend long distances. Quantitative analysis of counterstained preparations indicated that ChAT-positive neurons constitute 1.7% of the total neuronal population. Electron microscopy (EM) of immunoreactive neurons initially studied by LM revealed somata characterized by deeply invaginated nuclei and by abundant amounts of organelle-rich cytoplasm."
Comments P.29 left column 3rd paragraph: "Cholinergic interneurons were first identified as giant interneurons by Kölliker (1896). They are rare, accounting for only about 2% of the neurons in the mammalian striatum (primary sources)."
Entered by Uri M
ID 117206