Time after photobleaching that took gap junctional communication to recover

Range 1 - 4 hours after telophase
Organism Mammalian tissue culture cell
Reference Lampe PD, Lau AF. Regulation of gap junctions by phosphorylation of connexins. Arch Biochem Biophys. 2000 Dec 15 384(2):205-15 DOI: 10.1006/abbi.2000.2131 p.210 left column bottom paragraphPubMed ID11368307
Primary Source [99] Stein LS, Boonstra J, Burghardt RC. Reduced cell-cell communication between mitotic and nonmitotic coupled cells. Exp Cell Res. 1992 Jan198(1):1-7 DOI: 10.1016/0014-4827(92)90141-t [100] Xie H, Laird DW, Chang TH, Hu VW. A mitosis-specific phosphorylation of the gap junction protein connexin43 in human vascular cells: biochemical characterization and localization. J Cell Biol. 1997 Apr 7 137(1):203-10 DOI: 10.1083/jcb.137.1.203PubMed ID1727042, 9105048
Method FRAP (fluorescence recovery after photobleaching). Primary source [99] abstract: "The effects of mitosis on gap junctional intercellular communication (GJIC) were quantified in a clonal cell line of spontaneously immortalized rat granulosa cells (SIGC) using a fluorescence recovery after photobleaching assay." Primary source [100] abstract: "Western blotting studies revealed that connexin43 (Cx43), one of the major gap junction proteins in human vascular endothelial cells, is posttranslationally modified during mitosis."
Comments P.210 left column bottom paragraph: "Gap junctional communication is regulated during the progression of the cell cycle. Gap junctional communication, measuring dye spread via fluorescence recovery after photobleaching, was dramatically reduced in mitotic cells and gradually recovered between 1–4 h after telophase (primary sources)." Primary source [99] studied rat ovarian granulosa cell line and limited studies were conducted with an SV40-transformed monkey kidney cell line (COS-1, ATCC, Rockville, MD), a cell line derived from bovine coronary venular endothelial cells (CVRC) [ref 11 therein], an SV40-transformed rat granulosa cell line (SVGC), tumor-derived, SV40-transformed SIGC cells (T-SV-SICC)[ref 10 therein], and rat uterine myometrial cells. Primary source [100] studied Human umbilical vein endothelial cells (HUVEC), and Rat vascular smooth muscle cells.
Entered by Uri M
ID 117150