| Range |
in cultured (rat) hepatocytes 17 - 100: in mouse 3T3 cells >75 hours
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| Organism |
Mammalian tissue culture cell |
| Reference |
Lampe PD, Lau AF. Regulation of gap junctions by phosphorylation of connexins. Arch Biochem Biophys. 2000 Dec 15 384(2):205-15 DOI: 10.1006/abbi.2000.2131 p.206 left column bottom paragraphPubMed ID11368307
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| Primary Source |
[23] Chu FF, Doyle D. Turnover of plasma membrane proteins in rat hepatoma cells and primary cultures of rat hepatocytes. J Biol Chem. 1985 Mar 10 260(5):3097-107 [24] Hare JF, Taylor K. Mechanisms of plasma membrane protein degradation: recycling proteins are degraded more rapidly than those confined to the cell surface. Proc Natl Acad Sci U S A. 1991 Jul 1 88(13):5902-6 DOI: 10.1073/pnas.88.13.5902PubMed ID3972818, 2062868
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| Method |
Primary source [23] abstract: "The half-lives of turnover of plasma membrane proteins in rat hepatoma tissue, culture cells, and in primary cultures of rat hepatocytes have been analyzed after resolution by two-dimensional gel electrophoresis. Cell membranes were externally labeled via iodination catalyzed by lactoperoxidase and glucose oxidase… The turnover rates of membrane proteins of primary cultures of rat hepatocytes were also determined with [3H]- and [35S]methionine labeling of cells." Primary source [24] abstract: "Plasma membrane proteins of intact mouse 3T3 fibroblasts and H4-II-E-C3 hepatoma cells were separated into two groups based on their compartmentation between the cell surface and an intracellular compartment accessible at 20 degrees C but not at 0 degrees C. One group was derivatized at 0 degrees C with sulfosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate but not at 20 degrees C. The second group was derivatized at 20 degrees C as well as at 0 degrees C. Derivatized proteins were isolated from 35S-labeled cells on streptavidin/agarose and resolved by two-dimensional PAGE (polyacrylamide gel electrophoresis)." |
| Comments |
P.206 left column bottom paragraph: "These turnover rates [of Cx43 (Connexin43) and Cx45 (Connexin45), 1 - 3 hours, BNID 117146] are much faster than average rates for integral membrane proteins measured in cultured hepatocytes (t1/2=17–100h)(primary source 23) or mouse 3T3 cells (t1/2>75h)(primary source 24)." |
| Entered by |
Uri M |
| ID |
117148 |