Sodium (Na+) and potassium (K+) concentrations in cytosol

Range Na+ ~12: K+ ~150 mM
Organism Metazoa animals
Reference Haoxing Xu and Dejian Ren, Lysosomal Physiology, Annu. Rev. Physiol. 2015. 77: 57–80 doi: 10.1146/annurev-physiol-021014-071649 p.59 3rd paragraph
Primary Source [16] Steinberg BE et al., A cation counterflux supports lysosomal acidification. J Cell Biol. 2010 Jun 28189(7):1171-86. doi: 10.1083/jcb.200911083 [23] Wang X et al., TPC proteins are phosphoinositide- activated sodium-selective ion channels in endosomes and lysosomes. Cell. 2012 Oct 12 151(2):372-83. doi: 10.1016/j.cell.2012.08.036PubMed ID20566682, 23063126
Method Primary source [16] abstract: "To assess the individual contribution of counter-ions to acidification, [investigators] devised means of reversibly and separately permeabilizing the plasma and lysosomal membranes to dialyze the cytosol and lysosome lumen in intact cells, while ratiometrically monitoring lysosomal pH."
Comments P.59 3rd paragraph: "The luminal Na+ and K+ concentrations have been estimated in only a few cell types. The values range from 20 to 140 mM for Na+ (higher than [Na+]cytosol of ∼12 mM) and from 2 to 50 mM for K+ (lower than [K+]cytosol of ∼150 mM) (primary sources)."
Entered by Uri M
ID 117096