Intracellular alanine concentration with an isoosmolar extracellular environment

Value 50 mM
Organism Protozoa Giardia lamblia
Reference Adam RD. Biology of Giardia lamblia. Clin Microbiol Rev. 2001 Jul14(3):447-75 DOI: 10.1128/CMR.14.3.447-475.2001 p.455 left column 5th paragraphPubMed ID11432808
Primary Source [161] Knodler LA, Edwards MR, Schofield PJ. The intracellular amino acid pools of Giardia intestinalis, Trichomonas vaginalis, and Crithidia luciliae. Exp Parasitol. 1994 Sep79(2):117-25 DOI: 10.1006/expr.1994.1071 [270] Park JH, Schofield PJ, Edwards MR. Giardia intestinalis: volume recovery in response to cell swelling. Exp Parasitol. 1997 May86(1):19-28 DOI: 10.1006/expr.1996.4130PubMed ID8056076, 9149237
Method Primary source [161] abstract: "The total intracellular amino acid profiles of Giardia intestinalis trophozoites, Trichomonas vaginalis, and Crithidia luciliae were determined by sensitive amino acid analysis."
Comments P.455 left column 5th paragraph: "Alanine also appears to play an important role in allowing trophozoites to adapt to hypoosmotic challenge. With an isoosmolar extracellular environment, the intracellular alanine concentration is 50 mM (primary sources), and with a hypoosmolar challenge, the concentration of alanine rapidly decreases by an active transport mechanism."
Entered by Uri M
ID 117076