Photosynthetic carbon response parameters of Synechococcus spp. and Cyanobium spp.

Range Table - link
Organism Cyanobacteria
Reference Whitehead L, Long BM, Price GD, Badger MR. Comparing the in vivo function of α-carboxysomes and β-carboxysomes in two model cyanobacteria. Plant Physiol. 2014 May165(1):398-411. doi: 10.1104/pp.114.237941 p.402 table IIPubMed ID24642960
Method P.410 left column 5th paragraph: "Rubisco Kinetic Measurements-The Km values for RuBP [ribulose-1,5-bisphosphate] and CO2 were measured in Rubisco protein purified from Cyanobium spp. cell extracts and Synechococcus spp. PCC6301 Rubisco protein purified from E. coli cells. Assay conditions are detailed in the work by Whitney and Sharwood (2007) and are based on 14CO2 incorporation into PGA [phosphoglycerate]."
Comments P.400 right column top paragraph: "The response of both cell types to changes in external carbon was very similar in both low and high CO2 cells (Fig. 2, A and B). In both cell types, the affinity for external carbon increased markedly when cells were grown at low CO2 compared with high CO2 (Table II)." P.401 left column bottom paragraph: "Transmission electron microscopy images show that Synechococcus spp. cells were larger than Cyanobium spp. cells (Fig. 3). Chl [chlorophyll] and volume measurements in Table II confirm this observation." P.401 right column 2nd paragraph: "The Rubisco content of both species was measured in crude cell extracts from the stoichiometric binding of Rubsico’s tight-binding inhibitor 14CABP. The two strains had a very similar concentration of Rubisco (calculated from volume and active site density measurements, Table II)." P.405 left column bottom paragraph: "Both strains showed evidence of CCM [CO2-concentrating mechanism] induction when grown under low CO2, and in general, the photosynthetic characteristics of the two strains in response to light and external Ci [inorganic carbon] concentration were very similar (Figs. 1 and 2, Tables II and IV)."
Entered by Uri M
ID 117047