| Range |
~10 kb
|
| Organism |
Bacteriophage PM2 |
| Reference |
Krupovic M, Prangishvili D, Hendrix RW, Bamford DH. Genomics of bacterial and archaeal viruses: dynamics within the prokaryotic virosphere. Microbiol Mol Biol Rev. 2011 Dec75(4):610-35. doi: 10.1128/MMBR.00011-11 p.616 right column 3rd paragraphPubMed ID22126996
|
| Primary Source |
[97] Gray HB Jr, Upholt WB, Vinograd J. A buoyant method for the determination of the superhelix density of closed circular DNA. J Mol Biol. 1971 Nov 28 62(1):1-19 [174] Männistö RH, Kivelä HM, Paulin L, Bamford DH, Bamford JK. The complete genome sequence of PM2, the first lipid-containing bacterial virus To Be isolated. Virology. 1999 Sep 30 262(2):355-63 DOI: 10.1006/viro.1999.9837PubMed ID4333613, 10502514
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| Method |
Primary source [97] abstract: "A convenient method for the determination of the superhelix density of a closed circular DNA has been developed and tested with a series of closed DNA's with different superhelix densities and molecular weights. The method is based on the superhelix density-dependent binding affinity of closed DNA for the intercalating dye, ethidium bromide. The superhelix density is calculated from the observed separation between nicked and closed DNA in a cesium chlorideethidium bromide density gradient centrifuged under specified conditions in the preparative ultracentrifuge. The linear relation between the separations and the superhelix density obtained in this study is in good agreement with that predicted by Bauer & Vinograd (1970b).” Primary source [174] abstract: “Bacteriophage PM2 was isolated from the Pacific Ocean off the coast of Chile in the late 1960s. It was a new virus type, later classified as Corticoviridae, and also the first bacterial virus for which it was demonstrated that lipids are part of the virion structure. Here [investigators] report the determination and analysis of the 10,079-bp circular dsDNA genome sequence." |
| Comments |
P.616 right column 3rd paragraph : "Bacterial virus PM2 is the type member of the family Corticoviridae (ref 11). Its overall virion morphology resembles that of tectiviruses (Table 1), except that the genome of PM2 is a highly supercoiled circular dsDNA molecule of ∼10 kb (primary sources)." |
| Entered by |
Uri M |
| ID |
117000 |