| Value |
40
%
|
| Organism |
Unspecified |
| Reference |
Anastacio MM et al., Relationship between mitochondrial matrix volume and cellular volume in response to stress and the role of ATP-sensitive potassium channel. Circulation. 2013 Sep 10 128(11 Suppl 1):S130-5. doi: 10.1161/CIRCULATIONAHA.112.000128 p.S133 right column top paragraphPubMed ID24030396
|
| Primary Source |
[13] Katz AM. Physiology of the Heart. 2nd ed. New York, NY: Raven Press 1992: 23–28, 98–118 [24] Ganote CE, Armstrong SC. Effects of CCCP-induced mitochondrial uncoupling and cyclosporin A on cell volume, cell injury and preconditioning protection of isolated rabbit cardiomyocytes. J Mol Cell Cardiol. 2003 Jul35(7):749-59PubMed ID12818565
|
| Method |
Primary source [24] abstract: "The potential role of mitochondrial uncoupling and the resultant mitochondrial swelling, due to opening of the mitochondrial permeability transition pore (MPTP), were examined in an in vitro ischemically pelleted isolated rabbit cardiomyocyte model using the protonophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP) to uncouple mitochondria. Cyclosporin A (CsA) was employed to inhibit MPTP opening. Cell volume was determined by a cell-flotation, density-gradient assay, using bromododecane. Cell viability, subsequent to an osmotic stress, was determined by trypan blue permeability...Electron microscopy...[and] Morphometric analysis of the electron photomicrographs." |
| Comments |
P.S133 right column top paragraph: "The relationship between myocyte and mitochondrial volume is unknown. Other investigators have proposed relationships between the 2 volumes and have suggested that mitochondrial
swelling can comprise a significant portion of cell volume change and that the mitochondrial fraction increases to
40% of cytosolic volume in swollen cells [primary sources]." Primary source [24] studied rabbits |
| Entered by |
Uri M |
| ID |
116936 |