Diameter of over 95% of PAM (Pulmonary Alveolar Macrophages) prepared using cytocentrifuge procedures

Range 10 µm - 15 µm
Organism dog
Reference Haley PJ, Muggenburg BA, Weissman DN, Bice DE. Comparative morphology and morphometry of alveolar macrophages from six species. Am J Anat. 1991 Aug191(4):401-7 DOI: 10.1002/aja.1001910407 p.403 right column 2nd paragraphPubMed ID1951138
Primary Source Rebar AH, DeNicola DB, Muggenburg BA. Bronchopulmonary lavage cytology in the dog: normal findings. Vet Pathol. 1980 May17(3):294-304. doi: 10.1177/030098588001700303PubMed ID6154370
Method Primary source abstract: "Fiberoptic bronchoscopy was used to obtain cytologic specimens from all lung lobes of 9 normal Beagle dogs. Three specimen collection techniques (bronchial lavage, bronchial brushing and bronchial pinch biopsy imprints) and two staining procedures (Wright-Giemsa and Papanicolaou) were used and evaluated."
Comments P.403 right column 2nd paragraph: "The larger diameter reported here for the PAM of rats (18 µm) is probably a result of the cell spreading and flattening that occur during cytocentrifugation. Because of this flattening artifact, it is important to reproduce the methods described here when attempting to reproduce these findings. Diameters of cells in suspension would of course be expected to be much smaller. It has been reported (primary source) that more than 95% of the PAM of dogs prepared using similar cytocentrifuge procedures are 10-15 µm in diameter, values that are slightly less than those reported in this study."
Entered by Uri M
ID 116931