Reported diameters (3 measurements) of PAM (Pulmonary Alveolar Macrophages)

Range 10.5 µm: 11.5 µm: 9.8 µm
Organism Rat Rattus norvegicus
Reference Haley PJ, Muggenburg BA, Weissman DN, Bice DE. Comparative morphology and morphometry of alveolar macrophages from six species. Am J Anat. 1991 Aug191(4):401-7 DOI: 10.1002/aja.1001910407 p.403 right column 2nd paragraphPubMed ID1951138
Primary Source Davies P, Sornberger GC, Huber GL. The stereology of pulmonary alveolar macrophages after prolonged experimental exposure to tobacco smoke. Lab Invest. 1977 Sep37(3):297-306 AND Davies P, Sornberger GC, Engel EE, Huber GL. Stereology of lavaged populations of alveolar macrophages: effects of in vivo exposure to tobacco smoke. Exp Mol Pathol. 1978 Oct29(2):170-82PubMed ID197311, 689140
Method P.403 right column 2nd paragraph: "Davies et al. (1977 primary source) fixed rat PAM in glutaraldehyde-osmium, embedded them in Epon, and sectioned them at 1 µm." Primary source Davies et al. 1977 abstract: "Morphometric analysis was performed on alveolar macrophages obtained by bronchopulmonary lavage of rats exposed to tobacco smoke for periods up to 60 consecutive days…Electron microscopic stereology was used to quantitate alterations in the ultrastructure of the same macrophage preparations." Primary source Davies et al. 1978 abstract: "A multi-level morphometric and stereologic study was performed on alveolar macrophages obtained by bronchopulmonary lavage of unexposed control rats and rats experimentally exposed to whole tobacco cigarette smoke for 90 consecutive days. Measurements of macrophage profile diameters made at the light microscope were used to derive cell size distribution curves, with a mean cell volume in the control group of 623 μm^3."
Comments P.403 right column 2nd paragraph: "The PAM of rats had reported diameters of 10.5 µm, 11.5 µm, and 9.8 µm (primary sources)."
Entered by Uri M
ID 116930